Composite

Part:BBa_K2292006:Design

Designed by: FEI CHEN   Group: iGEM17_CCU_Taiwan   (2017-10-26)


MazEF kill switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

n/a


Source

We crafted our gene from sequences of mazE & mazF found in NCBI


References

1. PMC415763 - Escherichia coli mazEF-Mediated Cell Death Is Triggered by Various Stressful Conditions

2. PMC4333400 - Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding

3. doi: 10.1242/jcs.02619 - mazEF: a chromosomal toxin-antitoxin module that triggers programmed cell death in bacteria